Enhanced production of hyoscyamine and scopolamine from genetically transformed root culture of Hyoscyamus reticulatus L. elicited by iron oxide nanoparticles
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منابع مشابه
Quantitative Analysis of l-Hyoscyamine in Hyoscyamus reticulatus L. by GC-MS
The routine quality control of a herbal drug preparation requires the identification and determination of the contents of the active ingredients in the product. Measurement alone is not sufficient; it is also necessary to know how objectively to evaluate the experimental results . There are 6 Hyoscyamus (Solanaceae) species growing in Turkey, none of which are endemic. Hyoscyamus (Solanaceae) s...
متن کاملDetermination of Hyoscyamine and Scopolamine in Four Hyoscyamus Species from Iran
The purpose of the present study was to determine the tropane alkaloid content of four Hyoscyamus species from Iran, i.e. H. pusillus L., H. niger L., H. reticulatus L. and H. kurdicus Bornm. Determination of alkaloids was performed by the HPLC method. Samples were extracted with chloroform-methanol-25% ammonium hydroxide 15:5:1 (V/V/V). HPLC separation was performed on Eurospher C18 reversed p...
متن کاملDetermination of Hyoscyamine and Scopolamine in Four Hyoscyamus Species from Iran
The purpose of the present study was to determine the tropane alkaloid content of four Hyoscyamus species from Iran, i.e. H. pusillus L., H. niger L., H. reticulatus L. and H. kurdicus Bornm. Determination of alkaloids was performed by the HPLC method. Samples were extracted with chloroform-methanol-25% ammonium hydroxide 15:5:1 (V/V/V). HPLC separation was performed on Eurospher C18 reversed p...
متن کاملEffect of elicitation on antioxidant activity and production of tropane alkaloids in Hyoscyamus reticulatus hairy root cultures
Background and objectives: Hyoscyamus reticulatus contains two distinguished tropane alkaloids, hyoscyamineand scopolamine and both of the compounds possesspotential acute or chronic toxicity. In the present study, a simple and efficient transformation system was established for in vitro hairy roots induction in Hyoscyamus reticulatus. M...
متن کاملOriginal Contribution IN VITRO HYOSCYAMINE AND SCOPOLAMINE PRODUCTION OF BLACK HENBANE (HYOSCYAMUS NIGER) FROM SHOOT TIP CULTURE UNDER VARIOUS PLANT GROWTH REGULATORS AND CULTURE MEDIA
Tropane alkaloids, especially hyoscyamine (HYO) and scopolamine (SCO), are widely used in medicine for their antispasmodic, anticholinergic, and sedative properties. This study was undertaken to investigate the root and shoot tropane alkaloids production from in vitro shoot tip (as explant) culture of Hyoscyamus niger under different plant growth regulators including auxins (IAA, 2, 4-D, IBA, N...
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ژورنال
عنوان ژورنال: In Vitro Cellular & Developmental Biology - Plant
سال: 2017
ISSN: 1054-5476,1475-2689
DOI: 10.1007/s11627-017-9802-0